Roles of histone chaperone Nap1 and histone acetylation in regulating phase-separation of chromatin arrays.

ABSTRACT: Chromatin condensation is dynamically regulated throughout the cell cycle and plays key roles in modulating gene accessibility at the highest physical level in a cell. The DNA-histone dynamics in short- and long-range inter-nucleosomal interactions are central to the regulation mechanisms of chromatin condensation which remain under active investigation. We employed 12-mer nucleosome arrays to investigate the roles of histone chaperone Nap1, and histone H3 and H4 N-terminal tails and their acetylation in regulating the formation and the properties of phase-separated chromatin condensates. These arrays form liquid-like condensate droplets under a physiological salt condition of 150 mM NaCl. According to our results from bright-field microscopy, fluorescence recovery after photobleaching, op

SUBMITTER: Gao J 

PROVIDER: S-EPMC12132221 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature