Localization and function of septins are susceptible to epitope tagging.

ABSTRACT: Septins are hetero-oligomeric cytoskeletal proteins that assemble into filaments and scaffolds on the plasma membrane to aid cytokinesis, morphogenesis, and other cellular processes. Epitope tagging is widely used to study septin localization and function. However, functionality testing of tagged septins is often insufficient because of technical challenges. Fission yeast provides an ideal genetic system to test functionalities and localizations of tagged septins. mEGFP/mYFP tagged septins Spn1 and Spn4 localize exclusively to the division site as double rings during cytokinesis, but tdTomato tagged septins also localize to puncta or short linear structures across the plasma membrane. It was proposed that these additional septin structures serve as diffusion barriers and are important for

SUBMITTER: Gregory JR 

PROVIDER: S-EPMC12132285 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature