High-temporal-resolution point-of-care multiplex biomarker monitoring in small animals using microfluidic digital ELISA.

ABSTRACT: Time-course monitoring of blood biomarkers with rapid turnaround has the potential to revolutionize the diagnosis, stratification of phenotypes, and therapeutic/prognostic approaches for various acute inflammatory diseases in both clinical and preclinical studies. Current approaches, however, are hampered by slow turnaround times and large sample volume requirements, limiting the exploration of disease mechanisms and therapeutic strategies. Here, we developed a microfluidic digital ELISA platform prototype, combining single-molecule counting with whole blood assay capability for the first time from small animal models. This platform is automated and enables repeated, rapid biomarker monitoring with just 3.5 μL of whole blood collected from the tail. Our platform demonstrated high sensitivi

SUBMITTER: Song Y 

PROVIDER: S-EPMC12132392 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature