Application of post glycosylation modifying enzymes for mass spectrometry imaging of modified <i>N</i>-glycans <i>in situ</i>.

ABSTRACT: Glycans are essential components of cells and are involved in innumerable biological processes. Their structural diversity and complexity present unique analytical challenges. Glycans are comprised of various types of monosaccharides that are linked together at different positions and with varied stereochemistry. In addition, glycans are frequently decorated with a diverse set of chemical modifications, termed post-glycosylation modifications (PGMs). Characterization of PGMs is essential for a thorough understanding of glycans, however, the technical challenges and low throughput of current methodologies have limited our understanding of these modifications. Here we demonstrate a novel approach for rapid visualization of specific PGMs present in tissue N-glycans by applying PGM-targ

SUBMITTER: Dreifus JE 

PROVIDER: S-EPMC12132447 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature