MODELING TISSUE-RESIDENT MACROPHAGE DEVELOPMENT FROM MOUSE PLURIPOTENT STEM CELLS.

ABSTRACT: Tissue-resident macrophages (TRMs) are innate immune cells that participate in tissue development, homeostasis, and immune surveillance. Extensive efforts have been made to recapitulate TRM development from pluripotent stem cells (PSCs) in vitro to study molecular and cellular mechanisms of TRM development and to create cellular models of disease. However, available PSC models of mouse TRM development exhibit low overall efficiencies of TRM generation, produce heterogeneous off-target populations, and rely upon undefined media components, thus limiting their reproducibility, scalability, and widespread application as an experimental platform for TRM biology. To address these important limitations, we developed an efficient and reproducible protocol to faithfully recapitulate the ste

SUBMITTER: Baako AK 

PROVIDER: S-EPMC12132487 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature