α Protein Kinase 3 Is Essential for Neonatal and Adult Cardiac Function.

ABSTRACT:

Background

ALPK3 (α protein kinase 3) is an atypical kinase highly expressed in human and murine hearts. Biallelic loss-of-function mutations in ALPK3 lead to pediatric cardiomyopathy. The specific stages at which ALPK3 is essential for cardiac function and the mechanisms by which it regulates cardiac function require further exploration.

Methods and results

We generated ALPK3 global knockout and inducible cardiac-specific knockout mice. We performed time-course physiological and morphological assessments to determine ALPK3's role in neonatal and adult hearts. We also generated an Alpk3-3xFLAG-HA knock-in mouse model to determine endogenous ALPK3 localization. To investigate mechanisms of ALPK3 regulation, we performed biochemical assays and RNA sequencing experiments

SUBMITTER: Feng W 

PROVIDER: S-EPMC12132735 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature