Immune-epithelial-stromal networks define the cellular ecosystem of the small intestine in celiac disease.

ABSTRACT: The immune-epithelial-stromal interactions underpinning intestinal damage in celiac disease (CD) are incompletely understood. To address this, we performed single-cell transcriptomics (RNA sequencing; 86,442 immune, parenchymal and epithelial cells; 35 participants) and spatial transcriptomics (20 participants) on CD intestinal biopsy samples. Here we show that in CD, epithelial populations shifted toward a progenitor state, with interferon-driven transcriptional responses, and perturbation of secretory and enteroendocrine populations. Mucosal T cells showed numeric and functional changes in regulatory and follicular helper-like CD4⁺ T cells, intraepithelial lymphocytes, CD8⁺ and γδ T cell subsets, with skewed T cell antigen receptor repertoires. Mucosal changes remai

SUBMITTER: FitzPatrick MEB 

PROVIDER: S-EPMC12133578 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature