Chromosome-level genome assembly of a doubled haploid brook trout (Salvelinus fontinalis).

ABSTRACT: Brook trout (Salvelinus fontinalis) is a socioeconomically important fish species for fisheries, aquaculture, and aquatic conservation. We produced a 2.5-Gb reference assembly by combining Hi-C chromosome conformation capture with high-coverage short- and long-read sequencing of a fully homozygous mitotic gynogenic doubled-haploid fish, which facilitates the assembly of highly complex salmonid genomes. The assembly has an N50 of 50.98 Mb and 88.9% of the total assembled sequence length is anchored into 42 main chromosomes, of which 63.44% represents repeated contents, including 1,461,010 DNA transposons. 56,058 genes were found, with 98.6% of the 3,640 expected conserved orthologs BUSCO genes (actinopterygii_odb10 lineage database). Additionally, we found significant homology within the 42

SUBMITTER: Lecomte L 

PROVIDER: S-EPMC12134987 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature