Ninein isoform contributions to intracellular processes and macrophage immune function.

ABSTRACT: Ninein is a multifunctional protein involved in microtubule (MT) organization and dynein/dynactin complex recruitment and activation. Several isoforms of ninein have been identified in various tissues, however, their relative contribution(s) are not clear. Here, we identify two ninein isoforms in mouse macrophages with distinct C-termini and disproportionate expression levels; a canonical ninein (ninein^CAN) isoform and ninein isoform 2 (ninein^ISO2). Analysis of ninein pre-mRNA exon-intron boundaries revealed that ninein^ISO2 transcript is likely generated by two alternative splicing site selection events predicted to result in a distinct 3D structure compared to ninein^CAN. We used selective and total protein knockdown experiments to assess the int

SUBMITTER: Omer S 

PROVIDER: S-EPMC12135376 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature