Loss of sex-determining region Y-box 2 (Sox2) captures embryonic stem cells in a primed pluripotent state.

ABSTRACT: Two main pluripotent cell lines can be established from the preimplantation and postimplantation mouse embryo as naïve embryonic stem cells (ESCs) and primed epiblast stem cells (EpiSCs), respectively. Although the two pluripotent states are interconvertible, the molecular mechanism controlling the transition between naïve and primed pluripotency remains to be fully elucidated. Here, by performing a CRISPR-based loss-of-function screen in ESCs, we identify Sox2 involved in the repression of lineage-specification marker brachyury (T). Upon Sox2 ablation in ESCs, two populations of cells mutually exclusive for CDX2 (trophectoderm marker) and T expression can be observed. T-positive cells display features resembling the salient characteristics of EpiSCs including molecular and functional prop

SUBMITTER: Qi M 

PROVIDER: S-EPMC12135381 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature