Structure-based design of an immunogenic, conformationally stabilized FimH antigen for a urinary tract infection vaccine.

ABSTRACT: Adhesion of E. coli to the urinary tract epithelium is a critical step in establishing urinary tract infections. FimH is an adhesin positioned on the fimbrial tip which binds to mannosylated proteins on the urinary tract epithelium via its lectin domain (FimHLD). FimH is of interest as a target of vaccines to prevent urinary tract infections (UTI). Previously, difficulties in obtaining purified recombinant FimH from E. coli along with the poor inherent immunogenicity of FimH have hindered the development of effective FimH vaccine candidates. To overcome these challenges, we have devised a novel production method using mammalian cells to produce high yields of homogeneous FimH protein with comparable biochemical and immunogenic properties to FimH produced in E. coli. Next, to optimize confo

SUBMITTER: Silmon de Monerri NC 

PROVIDER: S-EPMC12136410 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature