Targeting Stat3 with conditional knockout or PROTAC technology alleviates renal injury by Limiting pyroptosis.

ABSTRACT:

Background

Acute kidney injury (AKI) is a critical clinical syndrome with high morbidity, mortality, and no effective treatment in clinical practice. The role of the Signal Transducer and Activator of Transcription 3 (Stat3) in AKI remains controversial, and its complex regulatory mechanisms must be further explored.

Methods

We generated renal tubular epithelial cells Stat3 conditional knockout (cKO) mice and used them in cecal ligation and puncture (CLP) and ischaemia-reperfusion (I/R) induced AKI models. Additionally, proteolysis-targeting chimaera (PROTAC) compound E034 was designed and synthesised. We also utilised human kidney tissues, mouse renal tubular epithelial cells (mTECs) and HK-2 cells for further studies, including immunohistochemistry, Western blot analysis,

SUBMITTER: Ji ML 

PROVIDER: S-EPMC12136849 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature