Molecular evidence of Wolbachia and Orthoflavivirus infection in field-collected mosquitoes in three provinces of Turkiye.

ABSTRACT:

Background

Mosquitoes transmit various pathogens causing diseases like Zika, Dengue, West Nile and Chikungunya. They also harbour insect-specific viruses (ISVs) and Wolbachia, which can block arbovirus transmission. This study investigated the prevalence of Orthoflavivirus and Wolbachia in mosquito populations from three provinces in Türkiye.

Methods

Mosquitoes were collected using CDC Miniature Light traps in 2022-2023. Morphologically identified specimens were pooled (1-10 individuals) and screened for Orthoflavivirus and Wolbachia via PCR and confirmed by Sanger sequencing. Infection prevalence was estimated using the maximum likelihood method. Mosquito taxa richness across provinces was estimated using the abundance-based, non-parametric Chao1 index.

Results

Among

SUBMITTER: Sewade W 

PROVIDER: S-EPMC12136952 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature