Investigating the use of cuprizone and lysolecithin to model demyelination <i>ex vivo</i> in sagittal rat brain organotypic slice cultures.

ABSTRACT:

Introduction

The development of organotypic slice cultures of central nervous system (CNS) tissues has bridged the gap between simple in vitro cell cultures and complex in vivo whole animal studies. Organotypic brain slice cultures are a useful tool to study neurological disease, providing a more complex 3-dimensional system than standard 2-dimensional in vitro cell culture. In particular, organotypic brain slice cultures provide an excellent model to study the processes of demyelination and remyelination associated with neurological disease and injury. However, organotypic brain slice cultures are typically generated using coronal sectioning or regionspecific hippocampal or cerebellar tissue. We have previously reported the ability to generate sagittal organotypic brain slice cult

SUBMITTER: Hawker B 

PROVIDER: S-EPMC12137318 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature