SnRNA-seq reveals subcutaneous white adipose tissue remodeling upon return to thermoneutrality after cold stimulation.

ABSTRACT:

Introduction

Cold stimulation induces browning of subcutaneous white adipose tissue (sWAT), making it a prime target for treating obesity and metabolic disorders. However, this remodeling is reversible: upon return to thermoneutrality (rewarming), sWAT whitens and loses its enhanced metabolic functions. Given the limited understanding of the microscopic dynamic changes and underlying mechanisms during this process, we established a temporally dynamic mouse model spanning the entire period from cold stimulation to the return to thermoneutrality, with inguinal sWAT (iWAT) selected as the study subject.

Methods

Based on preliminary data demonstrating stabilization in iWAT histology, expression levels of key thermogenic proteins, and the bulk transcriptome, we selected the two-w

SUBMITTER: Yang Y 

PROVIDER: S-EPMC12138199 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature