Expanding the Riboglow-FLIM Toolbox with Different Fluorescence Lifetime-Producing RNA Tags.

ABSTRACT: RNAs are essential elements of biology with subcellular localizations critical for function. Genetically tagging fluorescent RNA reporters to an RNA of interest allows for investigating RNA spatiotemporal dynamics. We previously developed a fluorescence lifetime imaging microscopy (FLIM)-based RNA-tagging platform, Riboglow-FLIM. Here, a genetically encoded RNA tag binds a fluorescent probe, causing an increase in both fluorescence intensity and fluorescence lifetime. Importantly, the Riboglow platform is derived from a bacterial riboswitch RNA family and different riboswitch sequences from nature may build the basis for multiplexing capabilities. We previously observed fluorescence lifetime differences for two RNA tags in vitro and in live mammalian cells as a proof-of-concept demo

SUBMITTER: Stickelman Z 

PROVIDER: S-EPMC12138973 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature