Hog1/p38 regulates the integrated stress response (ISR) and global transcriptomic changes in <i>C. neoformans</i> during oxidative stress.

ABSTRACT: Cryptococcus neoformans is an opportunistic fungal pathogen that is responsible for an estimated 112,000 deaths per year. We studied the role of the Hog1/p38 and Gcn2 signal transduction pathways during adaptation of C. neoformans to oxidative stress, a physiologically relevant stressor during infection. Using a combination of molecular assays and RNA-sequencing analysis in Hog1-, Gcn2- and double-deleted mutants, we identify shared targets of these pathways, and investigate a negative feedback loop that regulates induction of the integrated stress response (ISR). We found that simultaneous loss of Hog1 and Gcn2 profoundly impacts the ability of C. neoformans to tolerate oxidative stress. We also show that Hog1 regulates induction of the ISR, and that the Hog1 and Gcn2

SUBMITTER: Goich D 

PROVIDER: S-EPMC12139735 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature