Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naive chromosomal locus.

ABSTRACT: Maintaining genome integrity is crucial for the proper functioning and development of organisms. One intriguing aspect of genome integrity is the formation and function of neocentromeres at non-centromeric sites. CENP-A, a centromere-specific protein, is essential for centromere identification and function. However, in many cancers, CENP-A is often found to be ectopically misplaced when overexpressed. Moreover, CENP-A deposition at the centromere depends on the transcription of centromeric non-coding RNAs. Consequently, ectopic CENP-A is found at transcriptionally active and frequent breakpoint regions. To further explore ectopic CENP-A localization, we previously engineered a stable ectopic CENP-A site on a naïve chromosome by overexpressing a non-centromeric oncogenic lncRNA, PCAT2, whic

SUBMITTER: Sikder S 

PROVIDER: S-EPMC12139812 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature