Lineage recording in monoclonal gastruloids reveals heritable modes of early development.

ABSTRACT: Mammalian stem cells possess a remarkable capacity for self-organization, a property that underlies increasingly sophisticated in vitro models of early development. However, even under carefully controlled conditions, stem cell-derived models exhibit substantial "inter-individual" heterogeneity. Focusing on gastruloids, a powerful model of the early posterior embryo¹, we sought to investigate the origins of this heterogeneity. To this end, we developed a scalable protocol for generating gastruloids that are monoclonal, i.e. derived from a single mouse embryonic stem cell (mESC). Single cell transcriptional profiling of monoclonal gastruloids revealed extensive inter-individual heterogeneity, with some hardly progressing, others resembling conventional gastruloids b

SUBMITTER: Regalado SG 

PROVIDER: S-EPMC12139830 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature