Real-time dynamic monitoring and multiplex PCR identification of vector mosquitoes in Zhejiang, China.

ABSTRACT: The monitoring and identification of mosquito vectors are crucial for controlling the transmission of mosquito-borne diseases. Traditional mosquito morphological identification and surveillance methods, such as human landing catches, human-baited double net traps and BG-Sentinel mosquito traps, require a large amount of manpower but can only provide fragmented data. We utilized the MS-300, an internet-based vector mosquito monitor, to continuously capture and upload real-time data to cloud services across ten monitoring sites located in seven cities in Zhejiang Province, China from May to December 2023. A new multiplex PCR system was developed for amplifying the internal transcribed spacer 2 region, followed by employing both multiplex PCR and DNA barcoding techniques for detecting wild mo

SUBMITTER: He Q 

PROVIDER: S-EPMC12140415 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature