A multi-omics resource of B cell activation reveals genetic mechanisms for immune-mediated diseases.

ABSTRACT: Most genetic variants that confer risk of complex immune-mediated diseases (IMDs) affect gene regulation in specific cell types. Their target genes and focus cell types are often unknown, partially because some effects are hidden in untested cell states. B cells play central roles in IMDs, including autoimmune, allergic, infectious, and cancer-related diseases. Despite this established importance, B cell activation states are underrepresented in functional genomics studies. In this study, we obtained B cells from 26 healthy female donors and stimulated them in vitro with six activation conditions targeting key pathways: the B cell receptor (BCR), Toll-like receptor 7 (TLR7), TLR9, CD40, and a cocktail that promotes differentiation into double negative 2 (DN2) IgD^- CD27

SUBMITTER: Aguiar VRC 

PROVIDER: S-EPMC12140513 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature