Dendritic Morphology of Developing Hippocampal Neurons in Cyp11a1 Null Mice.

ABSTRACT:

Introduction

Neurosteroids have a variety of neurological functions, such as neurite growth, neuroprotection, myelination, and neurogenesis. P450scc, encoded by CYP11A1 gene, is the cholesterol side chain cleavage enzyme that catalyzes the first and rate-limiting step in steroidogenesis. In this study, we examine the dendritic morphology in developing hippocampal neurons of Cyp11a1 null mice at P15, a critical period for synapse formation and maturation.

Methods

Knockout mice were maintained until P15 with hormone administration. The Golgi-Cox method stained CA1 and CA3 pyramidal neurons in the hippocampus to reveal dendritic morphology.

Results

We demonstrated that Cyp11a1 null mice usually die within 7 days after birth and thus collected brain samples at postnatal d

SUBMITTER: Jiang HH 

PROVIDER: S-EPMC12140598 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature