Genetic Transformation of the Marine Oleaginous Microalga, Marinichlorella sp. NKG400014.

ABSTRACT: Microalgae attract significant attention as promising biofuel sources. However, lack of an efficient genetic transformation system hinders their use in advanced genetic engineering applications. We previously identified the marine oleaginous microalgal strain, NKG400014, from our marine microalgal culture collection via Nile red screening. Subsequent phylogenetic analysis revealed that strain NKG400014 belonged to the new genus, Marinichlorella. To date, successful genetic transformation of Marinichlorella species has not been reported. In this study, we aimed to investigate the growth characteristics of NKG400014 to clarify its oleaginous features as a biofuel producer. Then, we established a technique for NKG400014 genetic transformation via electroporation and optimized the key paramete

SUBMITTER: Kumakubo R 

PROVIDER: S-EPMC12141415 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature