Genomic and biochemical comparison of allelic triple-mutant lines derived from conventional breeding and multiplex gene editing.

ABSTRACT: Multiplex gene editing allows for the simultaneous targeting and mutagenesis of multiple loci in a genome. This tool is particularly valuable for plant genetic improvement, as plant genomes often require mutations at multiple loci to confer useful and/or novel traits. However, the regulation of gene editing can vary depending on the number of loci targeted. In this study, we developed triple-mutant soybean (Glycine max (L.) Merrill) lines using different crop improvement strategies, including conventional backcross breeding of standing variant alleles and clustered regularly interspaced short palindromic repeats-based multiplex editing to introduce new alleles. The mutations were targeted to genes encoding seed antinutritional components, as previously described in a triple null soybean ca

SUBMITTER: Liu J 

PROVIDER: S-EPMC12141651 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature