Interaction between 1-Cys peroxiredoxin and ascorbate in the response to H₂O₂ exposure in Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa, a leading cause of hospital-acquired infections, triggers host defenses, including oxidant release by phagocytes. Targeting bacterial antioxidants could reduce pathogen infectivity. This study investigates LsfA, a 1-Cys peroxiredoxin (Prx), member of the Prx6 subfamily, involved in P. aeruginosa virulence. LsfA efficiently reduced various peroxides (10⁶-10⁷ M^-1s^-1), while exhibiting hyperoxidation resistance (k_{hyperoxidation} ∼10² M^-1s^-1). Despite its substrate oxidizing promiscuity, LsfA displayed specific reduction by ascorbate (2.2 × 10³ M^-1s^-1). Moreover, elucidating the LsfA's crystallographic structures in the reduced and sulfinic/sulfonic ac

SUBMITTER: Aleixo-Silva RL 

PROVIDER: S-EPMC12141841 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature