Systematic bacteriophage selection for the lysis of multiple <i>Pseudomonas aeruginosa</i> strains.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen causing severe infections of the lung, burn wounds and eyes. Due to its intrinsic high antibiotic resistance the bacterium is difficult to eradicate. A promising therapeutic option is the use of P. aeruginosa-specific bacteriophages. Thus, the implementation of a phage therapy requires their selection, production and systematic administration using multiple strains of the bacterial target. Here, we used 25 phages and tested their susceptibility on 141 different P. aeruginosa strains isolated from patients with different types of infection. Comparative host spectrum analyses were carried out using double agar overlay plaque assay (DPA) and planktonic killing assay (PKA), which resulted in 70% of the cases in the same h

SUBMITTER: Rieper F 

PROVIDER: S-EPMC12141865 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature