Rapid CRISPR-Cas13a genetic identification enables new opportunities for listed Chinook salmon management.

ABSTRACT: Accurate taxonomic identification is foundational for effective species monitoring and management. When visual identifications are infeasible or inaccurate, genetic approaches provide a reliable alternative. However, these approaches are sometimes less viable (e.g., need for near real-time results, remote locations, funding concerns, molecular inexperience). In these situations, CRISPR-based genetic tools can fill an unoccupied niche between real-time, inexpensive, but error-prone visual identification and more expensive or time-consuming, but accurate genetic identification for taxonomic units that are difficult or impossible to visually identify. Herein, we use genomic data to develop CRISPR-based SHERLOCK assays capable of rapidly (<1 h), accurately (94%-98% concordance between phenotyp

SUBMITTER: Baerwald MR 

PROVIDER: S-EPMC12142720 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature