SysQuan: Repurposing SILAC Mice for the Cost-Effective Absolute Quantitation of the Human Proteome.

ABSTRACT: Relative quantitation, used by most mass spectrometry-based proteomics laboratories to determine protein fold-changes, requires samples being processed and analyzed together for best comparability through minimizing batch differences. This limits the adoption of mass spectrometry-based proteomics in population-wide studies and the detection of subtle but relevant changes in heterogeneous samples. Absolute quantitation circumvents these limitations and enables comparison of results across laboratories, studies, and over time. However, high cost of the essential stable isotope labeled (SIL) standards prevents widespread access and limits the number of quantifiable proteins. Our new approach, called "SysQuan", repurposes SILAC mouse tissues/biofluids as system-wide internal standards for matc

SUBMITTER: Mohammed Y 

PROVIDER: S-EPMC12143667 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature