Preclinical evaluation of glycan-targeting monoclonal antibodies for bimodal near-infrared fluorescence and photoacoustic imaging of gastrointestinal cancers.

ABSTRACT:

Background

Near-infrared fluorescence (NIRF) imaging assists surgeons intraoperatively to achieve radical resection of malignant tissue with one centimeter depth and can be supplemented with photoacoustic imaging to increase depth-of-view. Tumor-associated carbohydrate antigens are promising targets for tumor imaging with potential advantages over protein targeting. This study preclinically evaluates the anti-glycan tracers CH88.2-800CW (anti-Le^a/c/x) and CH129-800CW (anti-sdi-Le^a) for bimodal NIRF/PA imaging of gastrointestinal cancers.

Results

Using immunohistochemistry, we found that Le^a/c/x and sdi-Le^a were highly expressed in gastric and colorectal cancer tissue, with limited expression in healthy surrounding tissue, except fo

SUBMITTER: Houvast RD 

PROVIDER: S-EPMC12144009 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature