Next-gen novel nanocage-based multivalent vaccine candidate to tackle the rising menace of Mpox.

ABSTRACT: The recent emergence and global spread of the human Monkeypox virus (MPXV), including its transmission to non-endemic regions, have raised significant global health concerns. In this proof-of-concept study, we developed a recombinant protein-based MPXV vaccine candidate, employing an innovative and versatile multivalent, self-assembled nanocage protein scaffold. Two immunogenic antigens derived from the contemporary circulating MPXV strain have been incorporated into a self-assembled non-structural protein-10 (NSP-10) scaffold, expressed, and purified using an Escherichia coli expression system without a purification tag. The vaccine candidate elicited strong antibody responses in mice and conferred protection against the lethal Vaccinia virus in an intranasal and skin pock in vivo study.

SUBMITTER: Ahuja R 

PROVIDER: S-EPMC12144111 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature