CD44-downregulation in multiple myeloma inhibits cytoskeleton rearrangement through actin depolymerization.

ABSTRACT: It is well known that multiple myeloma (MM) cells are highly dependent on the bone marrow (BM) microenvironment. However, the complex interactions and signaling pathways between MM cells and BM stromal cells remain inadequately defined. In this study, we employed an in vitro coculture model to investigate these interactions. We found that coculturing MM cells with BM-derived HS5 stromal cells stimulated the secretion of hyaluronic acid (HA) and interleukin-6 (IL-6), and significantly increased the expression of CD44 and F-actin stress fibers polymerization in MM cells. Among the three hyaluronan synthase (HAS) isoforms, HAS3 mRNA expression was most significantly elevated in MM cells following coculture with HS5. This coculture also resulted in upregulation of HAS3 and IL-6 mRNA in MM cell

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12145313 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature