Functional regulation of macrophages by Ces1d-mediated lipid signaling in immunometabolism.

ABSTRACT:

Objective

Macrophage accumulation in metabolically active tissues during obesity is common in both animals and humans, but the lipid signaling mechanisms that trigger macrophage inflammation remain unclear. This study investigates the role of Ces1d, an unconventional lipase, in regulating macrophage inflammation under nutritional stress.

Methods

A myeloid-specific Ces1d knockout (LysM-Cre-Ces1d ^{floxed/floxed}, KO) mouse model was used for the studies. For in vitro tests, bone marrow-derived macrophages (BMDMs) from control (Ces1d ^{floxed/floxed}, WT) and KO mice were assessed for migration, polarization, and activation. For in vivo experiments, WT and KO mice were induced to obesity via a high-fat diet (HFD) and subjected to metabolic characterization. Adi

SUBMITTER: Shao LJ 

PROVIDER: S-EPMC12145999 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature