GSDMD is a novel predictive biomarker for immunotherapy response: in the pan-cancer and single cell landscapes.

ABSTRACT:

Background

Gasdermin D (GSDMD), a key executor of pyroptosis, has been implicated in modulating the tumor immune microenvironment. However, its role as a predictive biomarker for immunotherapy response remains unclear.

Methods

We conducted a pan-cancer analysis of GSDMD expression across TCGA datasets and investigated its association with tumor mutational burden (TMB), microsatellite instability (MSI), and mismatch repair (MMR) status. Immunological relevance was further assessed by correlating GSDMD expression with immune cell infiltration and immune checkpoint gene signatures. We performed single-cell RNA sequencing analysis to investigate the immune cell populations and immunological pathways associated with GSDMD expression. Finally, organoid-based functional assays conf

SUBMITTER: Huang LJ 

PROVIDER: S-EPMC12146313 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature