Modified pea apyrase has altered nuclear functions and enhances the growth of yeast and Arabidopsis.

ABSTRACT: Apyrases (NTPDases) regulate growth and development in multiple eukaryotic organisms and function in multiple sub-cellular locales. An earlier report showed that the ectopic expression of psNTP9 (PS), a chromatin-associated pea (Pisum sativum) apyrase, enhanced the uptake of inorganic phosphate (Pi) and increased the growth of yeast and Arabidopsis. In this follow-up study, we generated a modified form of PS, abbreviated DM ("double mutant"), in which two-point mutations, S208L and P216R, were introduced into its DNA-binding domain. Ectopic expression of DM increased the growth of yeast and Arabidopsis, the seed yield of Arabidopsis, and the Pi content of yeast and Arabidopsis grown in Murashige-Skoog media beyond that effected by PS. Both the PS and DM proteins co-purified with nuc

SUBMITTER: Tripathy MK 

PROVIDER: S-EPMC12146327 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature