Use of microarrays with different probe sizes for monitoring gene expression.

ABSTRACT: Microarrays with oligonucleotides of different lengths were used to monitor gene expression at a whole-genome level. To determine what length of oligonucleotide is a better alternative to PCR-generated probes, the performance of oligonucleotide probes was systematically compared to that of their PCR-generated counterparts for 96 genes from Shewanella oneidensis MR-1 in terms of overall signal intensity, numbers of genes detected, specificity, sensitivity, and differential gene expression under experimental conditions. Hybridizations conducted at 42 degrees C, 45 degrees C, 50 degrees C, and 60 degrees C indicated that good sensitivities were obtained at 45 degrees C for oligonucleotide probes in the presence of 50% formamide, under which conditions specific signals were detected by both PC

SUBMITTER: He Z 

PROVIDER: S-EPMC1214658 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature