Chromosome-segment scanning for gain- or loss-of-function screening (CHASING).

ABSTRACT: Identifying beneficial or functional mutations for a specific species is a task always relies on the labor-intensive construction of a library containing thousands of single-gene knockout or interference strains. Here, we systematically demonstrated that the task could be done by constructing a genome-scale library, designed by AutoGSL, containing a limited number of large fragment deletion strains. The loss-of- and gain-of-function phenotypes could be efficiently screened out by our chromosome-segment scanning, and the specific gene corresponding to an observed phenotype could be quickly identified and visualized by our computer-aided gene-annotation web tool. Additionally, a Clusters of Orthologous Gene Transformer learned representations were transferred to predict growth phenotypes of

SUBMITTER: Xia Y 

PROVIDER: S-EPMC12146616 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature