Viral and host network analysis of the human cytomegalovirus transcriptome in latency.

ABSTRACT: The human cytomegalovirus (HCMV) UL135 and UL138 genes play opposing roles regulating latency and reactivation in CD34⁺ human progenitor cells. We designed an RNA sequencing study to compare the transcriptional profile of HCMV infection in the presence and absence of these genes using the Tohoku Hospital Pediatrics-1 (THP-1) monocytic cell line model for latency. Relative to primary cell models, THP-1 cells offer the strength of a homogenous population that uniformly silences gene expression and will synchronously reexpress viral genes following stimulation to differentiate, which models early phases of viral reactivation. The loss of UL138 resulted in elevated levels of viral gene expression and in spontaneous adhesion of distinct cell populations that supp

SUBMITTER: Collins-McMillen D 

PROVIDER: S-EPMC12146695 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature