Virome Analysis Deciphers the First Virus Occurrence in Melothria scabra, Revealing Two Potyviruses, Including a Highly Divergent Zucchini Yellow Mmosaic Virus Isolate.

ABSTRACT: Melothria scabra, an annual vine plant belonging to the family Cucurbitaceae, is usually found as a weed in agricultural ecosystems, making it a potential reservoir for crop viruses. Nonetheless, no plant virus has been documented to infect M. scabra to date. In the present study, M. scabra leaves with plant virus disease symptoms were sampled and subjected to sequencing through metatranscriptome and small RNA methods. High-throughput data analysis revealed the presence of two potyvirus species, zucchini tigre mosaic virus (ZTMV) and zucchini yellow mosaic virus (ZYMV), which were subsequently confirmed through reverse transcription PCR (RT-PCR) detection. The complete genome sequences of ZTMV and ZYMV in M. scabra, designated as ZTMV-ms (PQ720520) and ZYMV-ms (PQ720521), were determined b

SUBMITTER: Zhu LJ 

PROVIDER: S-EPMC12146703 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature