Cardiolipin membranes drive Myosin VI activation, oligomerization, and processive cargo transport.

ABSTRACT: Mitochondrial damage determines cell fate, leading to mitochondrial autophagy or cellular apoptosis in health and disease. The molecular mechanisms and role of the acto-myosin cytoskeleton regulating mitochondrial clearance and membrane remodeling are critical in neurodegenerative disease progression including Alzheimer, but remain unclear. To investigate the potential link between full-length Myosin VI (FL-Myo6) recruitment and exposure of the mitochondria-specific lipid cardiolipin (CL), here we adapted a combination of molecular biology, biochemical, high-resolution fluorescence and interferometric light-scattering techniques. We developed analysis tools to reveal the structural Myo6-CL interaction sites, Myo6-oligomerization interfaces and mechanical properties. We found that CL activa

SUBMITTER: Montanarella AF 

PROVIDER: S-EPMC12146753 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature