High-affinity detection of biotinylated endogenous neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse.

ABSTRACT: Neuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs is hampered by the lack of efficient antibodies. Thus, to reveal their subcellular distribution, binding partners, and synaptic function, NLGNs were extensively manipulated using knock-down, knock-out, or overexpression approaches, leading to controversial results. As an alternative to the manipulation of NLGN expression level, we describe here the generation of a knock-in (KI) mouse strain in which native NLGN1 was N-terminally tagged with a small biotin acceptor peptide (bAP) that can be enzymatically biotinylated by the exogenous delivery of biotin ligase. After showing that KI mice exhibit normal

SUBMITTER: Ducrot C 

PROVIDER: S-EPMC12146770 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature