Integrated Analysis of Single-Cell and Bulk RNA-Sequencing Defines N7-Methylguanosine (m7G)-Mediated Modifications' Role in Prognosis and the Tumor Immune Microenvironment in Hepatocellular Carcinoma.

ABSTRACT:

Background

RNA 7-Methylguanosine (m7G) modification is biologically important to tumorigenesis and progression. Knowledge regarding the effect of m7G modification on the clinical features of hepatocellular carcinoma and its prognosis, tumor immune microenvironment, and sensitivity to immunotherapy remains limited.

Method

Single-cell RNA sequencing (scRNA-seq) data was sourced from the GEO repository. Transcriptomic datasets were procured from TCGA, GEO, and ICGC databases. Following this, we scrutinized 17 m7G-associated genes and executed unsupervised clustering to discern m7G modification profiles within HCC. Evaluation of immune cell infiltration levels and associated biological functions across varied m7G modification patterns was conducted using single-sample gene set e

SUBMITTER: Liang J 

PROVIDER: S-EPMC12146900 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature