Protein Kinase C-β Inhibition and Survival Signaling after Simulated Cardioplegic-Ischemia/Reperfusion in Nondiabetic and Diabetic Human Coronary Arterial Endothelial Cells.

ABSTRACT:

Background

Cardioplegic-ischemia/reperfusion (I/R) injury poses substantial challenges during postoperative recovery, with diabetic patients particularly susceptible to adverse events. Using a model entailing the subjection of human coronary artery endothelial cells (HCAECs) to simulated cardioplegic I/R, we investigated the potential of protein kinase C β (PKC-β) inhibition to augment cellular survival in this context.

Study design

HCAECs were isolated from harvested coronary arteries of diabetic (D) and nondiabetic (C) patients (N = 4 per group). HCAECs were either cultured under normoxic conditions without drug (D and C), subjected to hypoxia and reoxygenation alone (DH and CH), or subjected to hypoxia and reoxygenation and the PKC-β inhibitor LY333531 (DHT and CHT). Mole

SUBMITTER: Nho JW 

PROVIDER: S-EPMC12147101 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature