YTHDF2 suppresses the 2C-like state in mouse embryonic stem cells via the DUX-ZSCAN4 molecular circuit.

ABSTRACT: Mouse embryonic stem cells (ESCs) consist of a rare population of heterogeneous 2-cell-like cells (2CLCs). These cells transiently recapitulate the transcriptional and epigenetic features of the 2-cell embryos, serving as a unique model for studying totipotency acquisition and embryonic development. Accumulating evidence has demonstrated that transcription factors and epigenetic modifications exert crucial functions in the transition of ESCs to 2CLCs. However, the roles of RNA modification in the regulation of the 2C-like state remain elusive. Using a DUX-induced 2CLCs system, we examine N⁶-methyladenosine (m⁶A) modification landscape transcriptome-wide and observe dynamic regulation of m⁶A during DUX-driven 2C-like reprogramming. Notably, many core 2C tran

SUBMITTER: Wu X 

PROVIDER: S-EPMC12147224 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature