Detection of TuMV by a toehold switch sensor coupled with NASBA amplification in Pseudostellaria heterophylla.

ABSTRACT: Pseudostellaria heterophylla (P. heterophylla) is a perennial herb that has been used as a medicinal food for hundreds of years in China. Viral infections during the production of P. heterophylla severely reduce the yield and quality. Turnip mosaic virus (TuMV) is a common and highly variable viral pathogen in P. heterophylla plants. A high-efficiency diagnostic system is urgently needed to control and alleviate TuMV infection. However, the current detection methods still have various deficiencies that limit their field application. Here, a cell-free expression system relying on nucleic acid sequence-based amplification (NASBA) with toehold switch sensors and a visual reporter for color change was developed and introduced for TuMV detection in P. heterophylla. After designing and screening

SUBMITTER: Qiu Y 

PROVIDER: S-EPMC12147267 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature