Impact of respiratory syncytial virus on Streptococcus pneumoniae resistance to β-lactam antibiotics: an investigative study in children.

ABSTRACT:

Background

This study characterizes the molecular interplay between respiratory syncytial virus (RSV) glycoproteins (G/F) and Streptococcus pneumoniae (S.pn) penicillin-binding proteins (PBPs), while evaluating RSV's potential role in modulating S.pn β-lactam resistance.

Methods

Protein docking and pull-down assays assessed G/F-PBP interactions. In vitro RSV-S.pn co-culture experiments evaluated β-lactam susceptibility (MIC determination). We retrospectively analyzed 2012-2021 antimicrobial resistance data from 1-59-month-old community-acquired pneumonia patients at Chongqing Medical University Children's Hospital with confirmed S.pn and/or RSV nasopharyngeal carriage.

Results

Computational modeling revealed low G/F-PBP binding affinity (iPTM < 0.6), corroborated by a

SUBMITTER: Li L 

PROVIDER: S-EPMC12147270 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature