PorV factor of the type IX secretion system and PosF porin act as adhesins in Riemerella anatipestifer infection.

ABSTRACT: Riemerella anatipestifer infection is a critical disease that is a major threat to the poultry industry worldwide. The adhesion and invasion of host cells are key steps in the primary stages of bacterial infection. However, the outer membrane proteins that mediate these events in R. anatipestifer are poorly characterized. In this study, the PorV and PosF proteins, as well as the previously described OMP71 protein, were identified as important mediators of the adhesion and invasion of duck embryo fibroblast (DEF) cells by R. anatipestifer. Affinity chromatography-based surface proteomics was used to screen for adhesion proteins. The surface proteins on DEF cells were labelled with biotin-avidin to enrich for outer membrane proteins of R. anatipestifer, which generated 11 candidate proteins

SUBMITTER: Li S 

PROVIDER: S-EPMC12147357 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature