Proteome-Wide Assessment of Protein Structural Perturbations Under High Pressure.

ABSTRACT: One of the planet's more understudied ecosystems is the deep biosphere, where organisms can experience high hydrostatic pressures (30-110 MPa); yet, by current estimates, these subsurface and deep ocean zones host the majority of the Earth's microbial and animal life. The extent to which terrestrially relevant pressures up to 100 MPa deform most globular proteins - and which kinds - has not been established. Here, we report the invention of an experimental apparatus that enables structural proteomic methods to be carried out at high pressures for the first time. The method, called high-pressure limited proteolysis (Hi-P LiP), involves performing pulse proteolysis on whole cell extracts brought to high pressure. The resulting sites of proteolytic susceptibility induced by pressure are subse

SUBMITTER: Moran HM 

PROVIDER: S-EPMC12148218 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature