Mapping protein-metabolite interactions in <i>E</i>. <i>coli</i> by integrating chromatographic techniques and co-fractionation mass spectrometry.

ABSTRACT: Toward characterization of protein-metabolite interactomes, we recently introduced PROMIS, a co-fractionation-based mass spectrometry approach. However, the challenge lies in distinguishing true interactors from coincidental co-elution when a metabolite co-fractionates with numerous proteins. To address this, we integrated two chromatographic techniques-size exclusion and ion exchange-to enhance the mapping of protein-metabolite interactions (PMIs) in Escherichia coli. This integration aims to refine the PMI network by considering size and charge characteristics, resulting in 994 interactions involving 51 metabolites and 465 proteins. The PMI network is enriched for known and predicted interactions, providing validation. Furthermore, analyzing protein targets for different metabolit

SUBMITTER: Wagner M 

PROVIDER: S-EPMC12148380 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature