Evidence of bidirectional transmembrane signaling by the sensor histidine kinase GacS from Pseudomonas aeruginosa.

ABSTRACT: Membrane-embedded signaling histidine kinases (SKs) from two-component and phosphorelay signal transduction systems play central roles in the gene regulation of bacteria, fungi, and plants. The SK GacS is a global regulator of gene expression in the human pathogen Pseudomonas aeruginosa. The interactions between GacS and another SK, RetS, are a model for studying non-canonical crosstalk in multikinase networks. During planktonic growth, RetS inhibits GacS to upregulate expression of virulence factors associated with acute P. aeruginosa infections and repress genes linked to chronic infection. Conversely, GacS activation promotes biofilm formation and chronic infection but suppresses factors required during acute infection. Using a combination of hydrogen-deuterium exchange mass spectrometr

SUBMITTER: Salar S 

PROVIDER: S-EPMC12148439 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature