Serum Disease-Specific IgG Fc Glycosylation as Potential Biomarkers for Nonproliferative and Proliferative Diabetic Retinopathy Using Mass Spectrometry.

ABSTRACT: This study investigated the potential of serum disease-specific immunoglobulin G (DSIgG) crystallizable fragment (Fc) N-glycosylation as a diagnostic biomarker for the identification of nonproliferative and proliferative diabetic retinopathy (DR). A total of 160 patients were enrolled and categorized into three groups according to clinical diagnosis: non-diabetic retinopathy (NDR, n = 47); nonproliferative diabetic retinopathy (NPDR, n = 51); and proliferative diabetic retinopathy (PDR, n = 62). Gel electrophoresis was performed to separate IgG from morning fasting blood samples and polyaniline magnetic nanomaterials (Fe₃O₄@PANI) were used to enrich IgG N-glycopeptides from tryptic digestion. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry

SUBMITTER: Mao Y 

PROVIDER: S-EPMC12148564 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature